Journal: Advanced healthcare materials

Article Title: Designing Inherently Photodegradable Cell-Adhesive Hydrogels for 3D Cell Culture.

doi: 10.1002/adhm.202100632

Figure Lengend Snippet: Figure 2. A) Live/dead staining of normal human dermal fibroblasts (NHDF) with calcein-AM (green, live cells) and propidium iodide (red, dead cells) embedded in GelMA-free PEGMA/PEGDMA (Gel-N0) and GelMA-containing (Gel-N10) hydrogels on day 1, 7, 14 postencapsulation, followed by imaging with confocal microscopy (Leica TCS SPE, scale bar: 200 µm). B-i) NHDF embedded in Gel-N10 hydrogels on day 1 postencapsulation. Comparison of HepG2 cells encapsulated in (ii) Gel-N10 and (iii) Gel-N0 hydrogels (scale bar: 100 µm). C) Proliferation behavior of encapsulated HepG2 cells in Gel-N10 and Gel-N0 hydrogels over a period of 7 d assessed by PrestoBlue assay (n = 3). D) Toxicity evaluation of Gel-N10 photodegradation products by MTT proliferation assay with HeLa cells after 72 h exposure (n = 3). The concentration of nondiluted degradation products was 53 mg mL−1. Data are presented as mean ± SD and statistically evaluated by (C) Student’s t-test and (D) one-way ANOVA. *, **, *** represent p < 0.05, 0.01, and 0.001, respectively.

Article Snippet: Normal human dermal fibroblasts (NHDF), human liver cancer cells (HepG2), and HeLa cells were purchased from PromoCell GmbH (Heidelberg, Germany).

Techniques: Staining, Imaging, Confocal Microscopy, Comparison, Prestoblue Assay, Proliferation Assay, Concentration Assay

Journal: PLoS ONE

Article Title: Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls

doi: 10.1371/journal.pone.0108336

Figure Lengend Snippet: (a) Quantification of ABCC6 mRNA expression in human dermal fibroblasts from healthy controls (n = 5; white) and PXE patients (n = 6; black). Effect of siRNA-mediated knockdown on ABCC6 gene expression: fibroblasts transfected with a scramble siRNA-negative control (siNK, n = 3; white); ABCC6-specific siRNA-treated cells (siABCC6, n = 3; black). Expression levels are normalized to reference gene expressions (ACTB, GAPDH, ß2M). Data are presented in arbitrary units as means with corresponding standard error. Differences between controls and PXE fibroblasts, as well as between siRNA-transfected cells, were analyzed using unpaired t-test with Welch's correction. (b) Western Blot analysis of ABCC6 in fibroblasts of healthy controls, PXE patients and siRNA- treated cells. Western blot analysis was performed with pooled protein samples from each group, with GAPDH as normalization control. HEK-293 cell lysates were used as an additional blotting control. (c) Representative Western Blot for fibroblasts of healthy controls, PXE patients and (d) siRNA- treated cells.

Article Snippet: Dermal fibroblasts from six healthy controls (NHDF) were purchased from Promocell (Heidelberg, Germany), Genlantis (San Diego, USA), Cambrex (Walkersville, USA) and Coriell (New Jersey, USA).

Techniques: Expressing, Knockdown, Gene Expression, Transfection, Negative Control, Western Blot, Control

Journal: PLoS ONE

Article Title: Large-Scaled Metabolic Profiling of Human Dermal Fibroblasts Derived from Pseudoxanthoma Elasticum Patients and Healthy Controls

doi: 10.1371/journal.pone.0108336

Figure Lengend Snippet: Summary of metabolic findings in PXE human dermal fibroblasts compared to healthy controls.

Article Snippet: Dermal fibroblasts from six healthy controls (NHDF) were purchased from Promocell (Heidelberg, Germany), Genlantis (San Diego, USA), Cambrex (Walkersville, USA) and Coriell (New Jersey, USA).

Techniques:

Journal: BMJ Open Ophthalmology

Article Title: Protective effects of blue light-blocking shades on phototoxicity in human ocular surface cells

doi: 10.1136/bmjophth-2018-000217

Figure Lengend Snippet: Representative fluorescent microscopic images of immunostained cells with anti-pan-keratin antibody and its isotype control antibody (mouse IgG1). (A, B) Cells from human corneal surface origin (peripheral portion) and normal human dermal fibroblasts immunostained with anti-pan-keratin antibody, respectively. (C, D) Cells from human corneal surface origin (peripheral portion) and normal human dermal fibroblasts immunostained with an isotype control antibody (mouse IgG1), respectively. Scale bar=100 µm.

Article Snippet: Normal human dermal fibroblasts (NHDF, DS Pharma Biomedical, Osaka, Japan) were used as a negative control.

Techniques: Control